5J48
PKG I's Carboyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with 8-pCPT-cGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.510, 59.240, 67.320 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 34.952 - 1.490 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ku7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.253 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.240 |
| High resolution limit [Å] | 1.490 |
| Rmerge | 0.047 |
| Number of reflections | 44551 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 99.1 |
| Redundancy | 4.2 |
| CC(1/2) | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 400, 200 mM CaAc, 0.1M NaAc pH 4.5-4.8 |
