5J47

The X-ray structure of Inhibitor Bound to JCV Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	105
Detector technology	CCD
Collection date	2012-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999971
Spacegroup name	P 6
Unit cell lengths	110.710, 110.710, 67.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.960 - 1.990
R-factor	0.1851
Rwork	0.183
R-free	0.22100
RMSD bond length	0.009
RMSD bond angle	0.910
Data scaling software	SCALA (CCP4_3.3.20)
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.960
High resolution limit [Å]	1.990
Number of reflections	31960
<I/σ(I)>	16.8
Completeness [%]	98.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	25-30% Peg400, 0.1 M LiSO4, 0.1M HEPES (pH 7.0), 0.2 M NaCl