5J3N

C-terminal domain of EcoR124I HsdR subunit fused with the pH-sensitive GFP variant ratiometric pHluorin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	83.386, 176.505, 126.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.1949
Rwork	0.192
R-free	0.25383
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w7s
RMSD bond length	0.014
RMSD bond angle	1.729
Data reduction software	XDS (Oct 15, 2015)
Phasing software	MOLREP (11.4.04)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.450	2.450
Rmerge	0.101	0.510
Number of reflections	34464
<I/σ(I)>	11.7	2.74
Completeness [%]	99.2	97.4
Redundancy	4.68	4.63
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% (w/v) PEG 3350, 0.2 M KH2PO4, 4% v/v acetone