5J2U
Tubulin-MMAF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-15 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.567, 155.390, 182.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.654 - 2.500 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.24970 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.654 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 102228 | |
| <I/σ(I)> | 8.7 | 0.8 |
| Completeness [%] | 99.0 | 93.2 |
| Redundancy | 6.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% PEG, 12% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |
