5J2P
HIV-1 reverse transcriptase in complex with DNA that has incorporated EFdA-MP at the P-(post-translocation) site and a second EFdA-MP at the N-(pre-translocation) site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-10-02 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 165.977, 170.183, 99.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.780 - 2.530 |
| R-factor | 0.1959 |
| Rwork | 0.193 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 5J2M |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.518 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.780 | 63.780 | 2.620 |
| High resolution limit [Å] | 2.530 | 9.800 | 2.530 |
| Rmerge | 0.103 | 0.025 | 0.848 |
| Rmeas | 0.111 | 0.027 | 0.917 |
| Rpim | 0.041 | 0.010 | 0.346 |
| Total number of observations | 337771 | 6121 | 27230 |
| Number of reflections | 46900 | ||
| <I/σ(I)> | 16.7 | 56.2 | 2 |
| Completeness [%] | 98.9 | 99.4 | 88.6 |
| Redundancy | 7.2 | 6.7 | 6.7 |
| CC(1/2) | 0.998 | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.1 | 8% PEG4000, 25mM MES pH 6.0, 5mM magnesium sulfate |
