5J1S
TorsinA-LULL1 complex, H. sapiens, bound to VHH-BS2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.739, 90.659, 105.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.445 - 1.399 |
R-factor | 0.144 |
Rwork | 0.143 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4TVS_A |
RMSD bond length | 0.014 |
RMSD bond angle | 1.250 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 61.445 |
High resolution limit [Å] | 1.399 |
Number of reflections | 132851 |
<I/σ(I)> | 33 |
Completeness [%] | 93.0 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 13 % PEG 6000 5 % MPD 100 mM MES, pH 6.5 |