5J12

Structure of human TSLP:TSLPR in complex with mouse IL-7Ralpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	125.442, 125.442, 194.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.430 - 3.550
R-factor	0.2145
Rwork	0.214
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 4NN5
RMSD bond length	0.011
RMSD bond angle	1.190
Data reduction software	XDS (VERSION January 10, 2014)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.760
High resolution limit [Å]	3.550	3.550
Rmeas	0.177	1.122
Number of reflections	11341
<I/σ(I)>	10.8	2
Completeness [%]	98.4	97.9
Redundancy	10.7	10.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.53 M ammonium sulfate 0.1 M sodium chloride 0.1 M BIS-TRIS pH 7.0