5J01

Structure of the lariat form of a chimeric derivative of the Oceanobacillus iheyensis group II intron in the presence of NH4+ and MG2+.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 2
Unit cell lengths	100.930, 262.580, 69.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.755 - 3.390
R-factor	0.2057
Rwork	0.204
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4faw
RMSD bond length	0.012
RMSD bond angle	0.445
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.480
High resolution limit [Å]	3.390	15.150	3.390
Rmerge	0.315	0.053	4.762
Number of reflections	26308
<I/σ(I)>	11.67	62.22	0.68
Completeness [%]	99.7	95.8	96.9
Redundancy	28.1		17.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		301.15	50 mM Na-Cacodylate pH 6.5, 225 mM NH4Cl, 95 mM MgCl2, 22% MPD