5IXM
The LPS Transporter LptDE from Yersinia pestis, core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.892, 176.354, 143.848 |
| Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
| Resolution | 40.129 - 2.746 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.129 |
| High resolution limit [Å] | 2.746 |
| Rmerge | 0.150 |
| Number of reflections | 106219 |
| <I/σ(I)> | 8.4 |
| Completeness [%] | 99.3 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 50 mM sodium cacodylate pH 6.5, 10% polyethelyene glycol (PEG) 2000, 15% PEG 6000 |
