5IXI

Structure of human JAK1 FERM/SH2 in complex with IFNLR1/IL10RA chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000000000
Spacegroup name	C 1 2 1
Unit cell lengths	81.170, 110.420, 94.870
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	36.150 - 2.570
R-factor	0.2166
Rwork	0.213
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ixd
RMSD bond length	0.006
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.150	2.660
High resolution limit [Å]	2.570	2.570
Rmerge	0.083	0.732
Number of reflections	26423
<I/σ(I)>	13.8	2.2
Completeness [%]	99.4	98
Redundancy	3.7	3.8
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1M MES pH6.5, 0.2M MgCl2, 9% PEG4K, 25% ethylene glycol