5IXA

HCMV DNA polymerase processivity subunit UL44 at neutral pH and low salt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	.97919
Spacegroup name	P 21 21 21
Unit cell lengths	75.026, 100.829, 109.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.108 - 2.684
R-factor	0.2128
Rwork	0.210
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t6l
RMSD bond length	0.011
RMSD bond angle	1.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.108
High resolution limit [Å]	2.684
Number of reflections	23307
<I/σ(I)>	10.6
Completeness [%]	98.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.2 microliter of 8 mg/mL UL44 in the storage buffer (20 mM Tris (PH 7.5), 500 mM NaCl, 0.1 mM EDTA, 2 mM DTT and 5% glycerol) and 0.2 microliter crystallization buffer (0.1 M HEPES (PH 7.0) and 18% PEG12K) were mixed and crystallized in sitting drops over the crystallization buffer at room temperature