5IUE

Human leukocyte antigen F (HLA-F) presents peptides and regulates immunity through interactions with NK-cell receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 21 21 21
Unit cell lengths	102.453, 115.324, 168.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.140 - 2.620
R-factor	0.199
Rwork	0.197
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hsa
RMSD bond length	0.002
RMSD bond angle	0.676
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	95.144
High resolution limit [Å]	2.620
Rmerge	0.132
Number of reflections	60628
<I/σ(I)>	2.5
Completeness [%]	99.9
Redundancy	6.2
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 10000, tris-HCl