5ITZ
Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.080, 85.340, 98.690 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 52.644 - 2.200 |
| R-factor | 0.1789 |
| Rwork | 0.177 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4drx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.500 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.707 |
| Number of reflections | 51519 | |
| <I/σ(I)> | 18.11 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 550 mono methyl ether (MME) and 0.1 M MES, pH 6.5 |
