5IS8
Crystal structure of mouse CARM1 in complex with inhibitor SA0271
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.544, 98.623, 207.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.994 - 2.709 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.755 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2313: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 39433 | |
| <I/σ(I)> | 15.8 | 4.4 |
| Completeness [%] | 93.8 | 94.4 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | BTP pH 7.5 100 mM, PEG 3350 15% |
