5IRY

Crystal structure of human Desmocollin-1 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	213.806, 66.174, 141.633
Unit cell angles	90.00, 127.15, 90.00

Refinement procedure

Resolution	19.994 - 3.095
R-factor	0.2513
Rwork	0.249
R-free	0.30190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q2v
RMSD bond length	0.034
RMSD bond angle	1.071
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1810)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.994
High resolution limit [Å]	3.095
Number of reflections	28445
<I/σ(I)>	9.7
Completeness [%]	99.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% (w/v) PEG20000, 0.1M Tris-Bicine pH8.5, 5% (v/v) ethyleneglycol, 0.06M Calcium chloride, 20% (w/v) glycerol (cryoprotectant, added before freezing)