5IRY
Crystal structure of human Desmocollin-1 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.806, 66.174, 141.633 |
Unit cell angles | 90.00, 127.15, 90.00 |
Refinement procedure
Resolution | 19.994 - 3.095 |
R-factor | 0.2513 |
Rwork | 0.249 |
R-free | 0.30190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q2v |
RMSD bond length | 0.034 |
RMSD bond angle | 1.071 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1810) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.994 |
High resolution limit [Å] | 3.095 |
Number of reflections | 28445 |
<I/σ(I)> | 9.7 |
Completeness [%] | 99.1 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% (w/v) PEG20000, 0.1M Tris-Bicine pH8.5, 5% (v/v) ethyleneglycol, 0.06M Calcium chloride, 20% (w/v) glycerol (cryoprotectant, added before freezing) |