5IRM

Crystal structure of rabbit NOD2 in an ADP-bound state (Crystal form2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-19
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	112.153, 122.870, 177.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.310
R-factor	0.19511
Rwork	0.193
R-free	0.23681
RMSD bond length	0.007
RMSD bond angle	1.279
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	35238
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	5% PEG 10000, 100 mM Hepes pH 7.8, 500 mM NaCl