5INN

Mouse Tdp2 D358N protein, apo state with increased disorder amongst variable DNA-binding grasp conformations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	95.440, 114.880, 115.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.393 - 2.800
R-factor	0.2151
Rwork	0.213
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gz1
RMSD bond length	0.002
RMSD bond angle	0.577
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.113	0.052	0.459
Rmeas	0.129
Rpim	0.061
Total number of observations	127542
Number of reflections	30546
<I/σ(I)>	6.2
Completeness [%]	95.7	90.8	93
Redundancy	4.2	4.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	14-18% PEG3350, 100mM HEPES, 200mM lithium sulfate, 10mM magnesium acetate