5IN3
Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.760, 107.990, 126.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.290 - 1.730 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hxq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.062 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.800 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 86007 | 4211 |
| <I/σ(I)> | 1 | 0.5 |
| Completeness [%] | 99.8 | 62.7 |
| Redundancy | 9.4 | 10.4 |
| CC(1/2) | 0.996 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286 | 0.2M ammonium sulfate -- 30%(w/v) PEG 8000 |
