5ILQ
Crystal structure of truncated unliganded Aspartate Transcarbamoylase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-29 |
| Detector | DECTRIS PILATUS3 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.980, 103.800, 87.110 |
| Unit cell angles | 90.00, 117.68, 90.00 |
Refinement procedure
| Resolution | 45.030 - 2.500 |
| R-factor | 0.2082 |
| Rwork | 0.208 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml4 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.961 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.030 | 2.560 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.062 | 0.580 |
| Number of reflections | 45217 | |
| <I/σ(I)> | 10.81 | 1.98 |
| Completeness [%] | 95.2 | 95.6 |
| Redundancy | 2.51 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 200 mM Sodium Sulphate, 5 mM MgSO4, 15-18 % (w/v) PEG 3350 in 100mM bis-Tris Propane pH 7.5 |
