5IL1

Crystal structure of SAM-bound METTL3-METTL14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	102.344, 102.344, 116.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.710
R-factor	0.1811
Rwork	0.180
R-free	0.20690
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.904
Data scaling software	Aimless
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.710
Number of reflections	67836
<I/σ(I)>	17.7
Completeness [%]	100.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	291	PEG 8000, sodium citrate