5IL1
Crystal structure of SAM-bound METTL3-METTL14 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 102.344, 102.344, 116.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.710 |
R-factor | 0.1811 |
Rwork | 0.180 |
R-free | 0.20690 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.904 |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.000 |
High resolution limit [Å] | 1.710 |
Number of reflections | 67836 |
<I/σ(I)> | 17.7 |
Completeness [%] | 100.0 |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 291 | PEG 8000, sodium citrate |