5IIT
Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.640, 67.922, 129.677 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 46.859 - 2.134 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zr3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.748 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 101489 | |
| <I/σ(I)> | 15.5 | 1.9 |
| Completeness [%] | 99.1 | 96.7 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 19% PEG 3350, 0.1M AmSO4, 0.1M MES |
