5IIO

Crystal structure of the DNA polymerase lambda binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	191.423, 98.980, 105.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.086 - 2.080
R-factor	0.1923
Rwork	0.191
R-free	0.23510
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.090
High resolution limit [Å]	2.080
Number of reflections	118537
<I/σ(I)>	20
Completeness [%]	98.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.1 M KCl, 0.01 M CaCl2, 0.05 M HEPES pH 7.0, and 10% PEG 400