5IIM

Crystal structure of the pre-catalytic ternary extension complex of DNA polymerase lambda with an 8-oxo-dG:dA base-pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	56.274, 63.700, 140.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.947 - 1.941
R-factor	0.1866
Rwork	0.183
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsn
RMSD bond length	0.006
RMSD bond angle	0.898
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.030	1.947
High resolution limit [Å]	1.940	1.941
Rmerge	0.076	0.766
Number of reflections	36628
<I/σ(I)>	15.1	2.4
Completeness [%]	99.7
Redundancy	5.8
CC(1/2)	0.994	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.2 M ammonium acetate, 0.1 M sodium citrate pH 4.8, and 3-5% PEG 4000