5IHE

D-family DNA polymerase - DP1 subunit (3'-5' proof-reading exonuclease)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9919
Spacegroup name	P 21 21 21
Unit cell lengths	85.969, 91.415, 145.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.420 - 2.500
R-factor	0.1988
Rwork	0.197
R-free	0.22480
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	39885
<I/σ(I)>	6.9
Completeness [%]	98.9
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	277	100mM Na cacodylate pH6.7 200mM Ca Acetate 5% PEG8000