5IH3

Crystal structure of mouse CARM1 in complex with SAH at 1.8 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9304
Spacegroup name	P 21 21 2
Unit cell lengths	74.628, 97.920, 204.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 1.770
R-factor	0.1653
Rwork	0.164
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b3f
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.129	1.396
Number of reflections	146611
<I/σ(I)>	11.2	1.5
Completeness [%]	99.9	98.6
Redundancy	7.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Tris-HCl pH 8.0 100 mM, PEG 2000 MME 19%, NaCl 167 mM