5IGQ

WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2) bound to peptide from Trib1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Detector technology	CCD
Collection date	2014-04-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 2 2 2
Unit cell lengths	247.646, 249.576, 124.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.766 - 3.900
R-factor	0.2306
Rwork	0.228
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hqg
RMSD bond length	0.002
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.766	4.039
High resolution limit [Å]	3.900	3.900
Rmerge	0.069	0.733
Number of reflections	35006
<I/σ(I)>	8	1.2
Completeness [%]	98.0	100
Redundancy	2	2
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Bis-Tris pH 5.5, 20% glycerol, 5% PEG 5000 MME