5IF0
Crystal structure of VRC01c-HuGL2 Fab from an HIV-1 naive donor in complex with with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.44 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.192, 147.578, 103.685 |
| Unit cell angles | 90.00, 126.61, 90.00 |
Refinement procedure
| Resolution | 44.444 - 2.440 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jpk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.745 |
| Data reduction software | HKL-2000 (v706e) |
| Data scaling software | HKL-2000 (v706e) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.444 | 2.440 |
| High resolution limit [Å] | 2.408 | 2.408 |
| Rmerge | 0.778 | |
| Number of reflections | 73979 | |
| <I/σ(I)> | 9.2 | 1.1 |
| Completeness [%] | 95.2 | 94.2 |
| Redundancy | 2.8 | 2.5 |
| CC(1/2) | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M potassium dihydrogen phosphate, 20% (w/v) PEG3350 and cryo-protected with 25% glycerol |
