5ICW
Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.840, 84.298, 68.485 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 46.249 - 1.951 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lx9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.278 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.458 | 1.989 |
High resolution limit [Å] | 1.951 | 1.951 |
Rmerge | 0.098 | |
Number of reflections | 52859 | |
<I/σ(I)> | 12.82 | |
Completeness [%] | 94.6 | |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 3% Tascimate, 0.1 M Bistris pH 6.5 and 16% PEG 3350 |