5IC0
Structural analysis of a talin triple domain module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.007, 77.573, 61.622 |
| Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
| Resolution | 46.440 - 1.970 |
| R-factor | 0.20275 |
| Rwork | 0.201 |
| R-free | 0.23043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w8p 4f7g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.440 |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 29840 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 99.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH6.5, 21% (w/v) PEG 3350, 2% (w/v) benzamidine hydrochloride |
