5I69
MBP-MamC magnetite-interaction component mutant-D70A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.953 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.236, 67.236, 209.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.010 - 2.700 |
R-factor | 0.20859 |
Rwork | 0.204 |
R-free | 0.29284 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3puz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.584 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.010 | |
High resolution limit [Å] | 2.700 | |
Rmerge | 0.098 | 0.297 |
Number of reflections | 13928 | |
<I/σ(I)> | 44.02 | |
Completeness [%] | 90.3 | |
Redundancy | 16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M ammonium sulfate and 0.1M sodium citrate pH 4.5 |