5I2C
Arginine-bound CASTOR1 from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.393, 82.601, 96.666 |
| Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
| Resolution | 86.712 - 1.801 |
| R-factor | 0.1723 |
| Rwork | 0.172 |
| R-free | 0.20440 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.846 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.720 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 116806 | |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 0.1 M sodium acetate, 0.25 M ammonium acetate, 22.5% PEG 3350 |
