5HZV
Crystal structure of the zona pellucida module of human endoglin/CD105
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 125.160, 125.160, 88.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.504 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sex |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | XDS ((Jun 17, 2015)) |
| Data scaling software | XSCALE ((Jun 17, 2015)) |
| Phasing software | PHASER ((2.5.6)) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.510 | 2.760 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.675 | |
| Number of reflections | 21583 | |
| <I/σ(I)> | 9.43 | 0.99 |
| Completeness [%] | 97.9 | 85.4 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 11.5% equal mixture of MPD, PEG 1000 and PEG 3350 (1:1:1), MES/imidazole mix |
