5HZP

Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M49 protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	78.068, 78.068, 345.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.135 - 2.740
R-factor	0.2586
Rwork	0.255
R-free	0.31320
RMSD bond length	0.009
RMSD bond angle	1.219
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.135	2.890
High resolution limit [Å]	2.740	2.740
Rmerge		1.000
Number of reflections	29382
<I/σ(I)>	11.3	1
Completeness [%]	99.8	99.1
Redundancy	9.4	9.6
CC(1/2)	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	293	1.6 M Sodium Potassium Phosphate pH 6.9.