5HZN
Structure of NVP-AEW541 in complex with IGF-1R kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.435, 190.045, 155.488 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.786 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.078 | 0.054 | 0.413 |
| Number of reflections | 147785 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 90.9 | 99.5 | 78.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 25% PEG8000, 0.1M MES PH6.25, 0.2 M AMMONIUM SULFATE, 4% GLYCEROL |
