5HZJ

Crystal structure of photoinhibitable Intersectin1 containing wildtype LOV2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97903
Spacegroup name	P 1 21 1
Unit cell lengths	61.650, 111.170, 88.190
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	47.024 - 2.600
R-factor	0.253
Rwork	0.252
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ki1 2wkq
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.059	0.453
Number of reflections	35551
<I/σ(I)>	12.5	2.6
Completeness [%]	97.0	97.2
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M HEPES pH 7.5, 9% (w/v) PEG 8000, 9% (v/v) ethylene glycol