5HYP

Structure of human C4b-binding protein alpha cain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M28 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 3 2
Unit cell lengths	133.192, 133.192, 133.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.397 - 3.024
R-factor	0.2549
Rwork	0.251
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hyu
RMSD bond length	0.009
RMSD bond angle	1.307
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.397	3.070
High resolution limit [Å]	3.020	3.020
Rmerge	0.140	1.000
Number of reflections	8361
<I/σ(I)>	13.2	0.81
Completeness [%]	99.8	100
Redundancy	9.5	9.7
CC(1/2)	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	1.5 M Ammonium Sulfate, 0.1 M Bis-Tris Propane