5HYP
Structure of human C4b-binding protein alpha cain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M28 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 133.192, 133.192, 133.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.397 - 3.024 |
| R-factor | 0.2549 |
| Rwork | 0.251 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hyu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.397 | 3.070 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.140 | 1.000 |
| Number of reflections | 8361 | |
| <I/σ(I)> | 13.2 | 0.81 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.5 | 9.7 |
| CC(1/2) | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.5 M Ammonium Sulfate, 0.1 M Bis-Tris Propane |
