5HYN

Structure of Human Polycomb Repressive Complex 2 (PRC2) with oncogenic histone H3K27M peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	131.640, 171.510, 274.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.013 - 2.950
R-factor	0.2218
Rwork	0.219
R-free	0.27330
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.523
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.430	3.020
High resolution limit [Å]	2.940	2.940
Rmerge	0.165	1.607
Number of reflections	132394
<I/σ(I)>	9.9	1.4
Completeness [%]	99.9	99.9
Redundancy	5.6	5.6
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	25% PEG3350, 400mM Ammonian Citrate pH6.5