5HXA

Crystal structure of an UDP-forming alpha, alpha-terhalose-phosphate synthase from Burkholderia xenovorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-10
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 64 2 2
Unit cell lengths	171.180, 171.180, 95.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.280 - 2.000
R-factor	0.158
Rwork	0.157
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wtx
RMSD bond length	0.007
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.454
Number of reflections	55408
<I/σ(I)>	18.26	4.52
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	MCSG1 A8 (267517a8): 100mM Tris:HCl pH7.0, 20% (w/v) PEG2000MME, 3mM UDP-glucose, 3mM glucose-6-phosphate, protein conc. 20mg/mL, 20% ethylene glycol cryoprotectant, puck hnk1-2