5HVJ
Crystal structure of LIMK1 D460N mutant in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.987, 59.573, 98.276 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 39.979 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s95 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.979 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.156 | |
| Number of reflections | 31929 | |
| <I/σ(I)> | 12.8 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18-24% PEG 3350, 0.2 M potassium citrate |
