5HR6
X-ray crystal structure of C118A RlmN with cross-linked tRNA purified from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.664, 69.821, 149.298 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 88.663 - 2.880 |
| R-factor | 0.18365 |
| Rwork | 0.182 |
| R-free | 0.22811 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 88.663 | 88.663 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
| Rmerge | 0.132 | 0.066 | |
| Number of reflections | 23364 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.6 | 99.2 | 99.6 |
| Redundancy | 6.3 | 5.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, potassium chloride, magnesium chloride, Tris-HCl |
