5HPY

Crystal Structure of RhoA.GDP.MgF3-in complex with human Myosin 9b RhoGAP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	114.055, 90.762, 93.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.698 - 2.400
R-factor	0.2126
Rwork	0.211
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ow3
RMSD bond length	0.009
RMSD bond angle	1.257
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	38652
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	20% (w/v) PEG 3350, 0.2M sodium malonate pH 7.0.