5HNS
Structure of glycosylated NPC1 luminal domain C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0675 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.890, 115.910, 147.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.030 - 2.450 |
| R-factor | 0.22474 |
| Rwork | 0.224 |
| R-free | 0.23816 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.519 |
| Data reduction software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.000 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 28062 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 100.0 |
| Redundancy | 26.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 30% polyethylene glycol mono-ethyl Ether 2000 and 0.1 M potassium thiocyanate |
