5HLY

Structure of Pro-Activin A Precursor at 2.3 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97866
Spacegroup name	P 61 2 2
Unit cell lengths	46.561, 46.561, 444.921
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.150 - 2.302
R-factor	0.2049
Rwork	0.203
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	experimental phases from separate dataset
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2084: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.150	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.107	0.687
Number of reflections	11247
<I/σ(I)>	17.3	2.4
Completeness [%]	79.3	37.1
Redundancy	14.3	7.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	25% w/v polyethylene glycol 1000, 100 mM MES pH 6.5; cryo: 15% v/v PEG 400 added