5HLY
Structure of Pro-Activin A Precursor at 2.3 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97866 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.561, 46.561, 444.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.150 - 2.302 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | experimental phases from separate dataset |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.724 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2084: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.150 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.687 |
| Number of reflections | 11247 | |
| <I/σ(I)> | 17.3 | 2.4 |
| Completeness [%] | 79.3 | 37.1 |
| Redundancy | 14.3 | 7.3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 25% w/v polyethylene glycol 1000, 100 mM MES pH 6.5; cryo: 15% v/v PEG 400 added |
