5HH3
OxyA from Actinoplanes teichomyceticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.07169 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.490, 103.060, 152.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 2.100 |
| R-factor | 0.19969 |
| Rwork | 0.198 |
| R-free | 0.22859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ex8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.850 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.744 |
| Number of reflections | 54785 | |
| <I/σ(I)> | 20 | 4.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Mes/ NaOH pH 6.5, 14.4% (w/ v) PEG 8,000, 20% (v/ v) glycerol, 0.16 M calcium acetate |
