5HH1

Crystal structure of human Naa60 mutant - F34A in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	P 21 21 2
Unit cell lengths	66.685, 74.031, 43.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.521 - 1.803
R-factor	0.1898
Rwork	0.189
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hgz
RMSD bond length	0.015
RMSD bond angle	1.581
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.026	0.478
Number of reflections	20501
<I/σ(I)>	27.9	2.4
Completeness [%]	99.9	99.7
Redundancy	6.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8.5	293.15	0.2M Lithium Sulfate monohydrate, 0.1M Tris pH 8.5, 20%(w/v) PEG3350