5HGJ
Structure of integrin alpha1beta1 and alpha2beta1 I-domains explain differential calcium-mediated ligand recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.369, 95.950, 53.078 |
Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
Resolution | 47.975 - 1.399 |
R-factor | 0.1789 |
Rwork | 0.178 |
R-free | 0.20510 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 150.000 |
High resolution limit [Å] | 1.360 |
Number of reflections | 67058 |
<I/σ(I)> | 14.7 |
Completeness [%] | 94.6 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | PEG 8000, Tris-HCl, Calcium Chloride, Sodium Chloride, Glycerol |