5HE0

Bovine GRK2 in complex with Gbetagamma subunits and CCG215022

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	60.554, 239.927, 208.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.560
R-factor	0.2048
Rwork	0.202
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pnk
RMSD bond length	0.013
RMSD bond angle	1.646
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.640
High resolution limit [Å]	2.600	7.030	2.600
Rmerge	0.067	0.023	0.391
Rmeas	0.078	0.028	0.476
Rpim	0.040	0.015	0.268
Total number of observations	162451
Number of reflections	47002
<I/σ(I)>	12.6
Completeness [%]	97.0	95.1	86.1
Redundancy	3.5	3.4	2.5
CC(1/2)		0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES, 0.8-1.2 M sodium chloride, 8-16% PEG3350