5HBB
Crystal structure of shaft pilin spaA from Lactobacillus rhamnosus GG - E139A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 223.950, 63.488, 104.453 |
Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
Resolution | 111.800 - 2.470 |
R-factor | 0.2013 |
Rwork | 0.200 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f44 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.800 | 2.554 |
High resolution limit [Å] | 2.470 | 2.466 |
Rmerge | 0.089 | 0.347 |
Number of reflections | 44441 | |
<I/σ(I)> | 17.36 | 3.84 |
Completeness [%] | 83.5 | 27.02 |
Redundancy | 5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M sodium thiocynate, 15% PEG3350 |