5HAB
Crystal structure of mpy-RNase J (mutant H84A), an archaeal RNase J from Methanolobus psychrophilus R15, complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-18 |
| Detector | PHILLIPS |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 169.680, 169.680, 167.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.210 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.937 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1980) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.840 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.105 | 0.919 |
| Number of reflections | 63078 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.8M ammonium sulfate, 3% glycerol |
