5H7S

Structural basis of the flanking zinc-finger motifs crucial for the E3 ligase activity of the LNX1 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	PIXEL
Collection date	2016-07-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	122.372, 122.372, 135.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	105.980 - 3.490
R-factor	0.24547
Rwork	0.241
R-free	0.28649
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5din 3hct 1ubq
RMSD bond length	0.010
RMSD bond angle	1.536
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0171)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.950	3.810
High resolution limit [Å]	3.480	3.480
Number of reflections	15353
<I/σ(I)>	16.1
Completeness [%]	99.8
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	0.04 M Citric acid, 0.06 M BIS-TRIS propane, pH 6.4, 20% w/v polyethylene glycol 3350